Fig. 1. Cryptocyanin and hemocyanin protein levels in the hemolymph during sequential molt cycles of Cancer magister. Instar times expressed as % molt cycle, adjusted to average duration for that instar stage (2nd instar molt cycle averaged 16 days, while 6th instar averaged 30 days). Hemolymph samples were run on non-denaturing, non-dissociating pH 7.4 PAGE. Protein concentrations were determined from image analysis of gels using standard curves of purified hemocyanin and cryptocyanin of C. magister. Data expressed as means ±1 S.E.M. (2nd instar, N=13; 3rd instar, N=23; 4th instar, N=23; 5th instar, N=16; 6th instar, N=5). Arrows indicate ecdysis.