Fig. 1. Isolation of a single, identifiable neuron from the Lymnaea CNS. (A) Ventral view of the right cerebral ganglion in L. stagnalis. The arrow indicates the cerebral giant cell. Scale bar, 100 µm. (B) The same cerebral ganglion after isolation of the cerebral giant cell. (C) Light microscopic photograph of the isolated cerebral giant cell. Scale bar, 50 µm. (D) Hoechst staining of the same cell. (E) Merged image of (C) and (D). Only the nucleus of the isolated, single cerebral giant cell was observed, indicating that no other cells were removed along with the cerebral giant cell.