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Fig. 6. Rh2 opsins. (A) Deduced amino acid sequences from four species of
notothenioid fish (GenBank accession numbers AY771352-AY771355). Residue 122
is boxed. (B) Neighbor-joining tree of opsin amino acid sequences. The numbers
at each branch point are the bootstrap values derived from 1000 bootstraps.
The scale bar is calibrated as 0.1 substitutions per site, with the length of
each branch proportional to divergence. Drosophila Rh3 (GenBank
accession number NM_079687) was used as an outgroup. Goldfish opsin GenBank
accession numbers: Rh1 L11863, Rh2 L11866, SWS1 D85863, SWS2 11864, LWS L11867
(C) In vitro absorbance spectrum for the pigment from D.
mawsoni. The difference spectrum shown with a fitted Govardovski visual
pigment template, was obtained by subtracting the bleached spectrum from the
dark spectrum.
