Fig. 7. UV opsin-ir (Alexa Fluor 488, green or Cy3, red) in perikarya along the edge of the medulla (Me) and the protocerebrum (Pr), within the central body (CB) and antennal lobes (AL), and PER-ir in the AL. Nuclei are stained with DAPI (blue). (A) UV opsin-like ir was found in a cluster of approximately 10 perikarya located at the ventral edge of the medulla (arrow). Arrowhead indicates autofluorescing cuticle. (B) An adjacent section to A except that the antibody was incubated prior to application with the peptide against which it was raised for 1 h at room temperature (see Materials and methods). Arrow indicates the location of perikarya in which the UV opsin-ir is undetectable. (C) Sketch of a posterior section through the optic lobe of a worker bee showing the location of the UV opsin-ir perikarya (red asterisk) shown in A. (D,E) UV opsin-ir (Cy3, red) of a cell cluster between the dorsal-median rim of the lobula (Lo) and the lateral calyces of the mushroom bodies. These cells are in a similar location to pigment dispersing hormone (PDH)-ir cells in the honeybee (Bloch et al., 2003). The arrow in D is pointing to the cell cluster and in E to an individual cell. (F) Sketch of an anterior section of a worker bee brain showing the location of the UV opsin-ir cell cluster (red asterisk) shown in D and E, which is located between the anterior lateral protocerebrum and the optic lobe. (G) Frontal section of the antennal lobe. Strongest UV opsin-ir was found in the core region of each glomeruli. (H) UV opsin-ir (Alexa Fluor 488, green) was found in the upper part of the central body. (I) Adjacent section to H, processed with the primary antibody omitted. (J) PER-ir in the deuterocerebrum. Strongest labeling was found in the lateral cell cluster (arrow). These perikarya belong to local interneurons and projection neurons (Rybak and Menzel, 1993). Dorsal and ventral clusters exhibit weaker labeling. (K) Same section as in J, showing nuclei of all antennal lobe cell bodies labeled by DAPI (blue). CB(u), central body, upper region; CB(l), central body, lower region; Lo, lobula; Me, medulla; Pr, protocerebrum; OL, optic lobe; lCa, lateral calyx; mCa, median calyx; D, dorsal; V, ventral. Scale bars, 20 µm (A,B,E, G,H,I); 50 µm (D,J,K).