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Fig. 2. Phylogeny of insect UV opsins based on 1st and 2nd nucleotide positions and the Tajima-Nei model of evolution with complete deletion of gaps. The A. mellifera blue opsin sequence was used as an outgroup. Bootstrap values based on 500 replications are shown above nodes for 1st and 2nd positions and below nodes for amino acids (italics). Branch length indicates nucleotide substitutions per site. GenBank accession numbers for sequences used in our analysis are as follows: Anopheles gambiae GPRop8 (AAAB01008960 gi|19612143: 3070327-13075141), Apis mellifera (UV, AF004169; blue, AF004168), Bicyclus anynana (AF484248), Camponotus abdominalis (U32502), Cataglyphis bombycina (U32501), Drosophila melanogaster (Rh3, M17718; Rh4, M17719), Drosophila pseudoobscura (Rh3, X65879; Rh4, X65880), Manduca sexta (L78081), Megoura viciae (AF189715), Papilio glaucus (AF077191), Papilio xuthus (AB028218).