Fig. 7. Worms adapt to the loss of nicotinic MC stimulation by altering resting potential to preserve pharyngeal pumping. (A) Sample intracellular voltage recordings from wild-type worms show that resting potential is about -73 mV. (B) Resting potential in cca-1 single mutants matches that of wild-type worms. (C) In contrast, a recording from an eat-2 mutant reveals that resting potential is elevated by 13 mV over wild-type resting potential, and has a slight tendency to rise between action potentials. (D) A recording from an eat-2; cca-1 double mutant reveals an elevated resting potential that drifts significantly towards positive potentials between action potentials. (E) Average resting membrane potential for above four strains. Mean ± S.E.M.; N=12 (WT), 10 (cca-1), 15 (eat-2), 11 (eat-2; cca-1). ^*Significantly different from both the wild type and cca-1 (P<0.005, t-test).