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Fig. 3. Expression of HvAPX1 in symbiotic and aposymbiotic polyps. (A) RT-PCR. The quantity of the cDNA template was equalized using Hydra viridis actin specific primers. (B) Northern blot hybridisation of 8 µg total RNA from asexual, testes-bearing and hermaphroditic polyps as well as 4 µg of total RNA from symbiotic Chlorella with radiolabelled HvAPX1 revealing a transcript of about 1300 bases. Lower panel shows the amount of ribosomal RNA within the blotted samples. s, symbiotic polyps; a, aposymbiotic polyps; Chl, symbiotic Chlorella.