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Fig. 3. Interactions between CSTX-9 and CSTX-1, or cupiennin 1a as measured in a Drosophila bioassay. (A) The mortality caused by CSTX-1 alone (filled bar; 5.99 µmol l-1) was compared with the mortality due to co-injection with CSTX-9 (open bar; 1.05 µmol l-1; n.s.) corresponding to their physiological venom ratio. The mortality caused by cupiennin 1a alone (filled bar; 90 µmol l-1) was compared with the mortality due to co-injection with CSTX-9 (filled bar; 50 µmol l-1; n.s.). CSTX-9 showed no toxic effect when administered alone (control, not shown). (B) The mortality caused by CSTX-1 alone (open bar; 8.99 µmol l-1) or CSTX-9 alone (hatched bar; 143.14 µmol l-1), or their theoretical sum respectively, was compared with the mortality due to the co-injection of CSTX-1 (8.99 µmol l-1) and CSTX-9 (effective sum; 143.14 µmol l-1; {dagger}P<0.001). All data with mean ±S.E.M. (N=24 groups); Fisher LSD test.