Fig. 4. (A) CnA nuclear localisation was significantly higher in the E₁₅ and E₃₀ groups relative to the TR group (P<0.001, one-way ANOVA; ^**P<0.01, ^***P<0.001, Tukey's test). Overall CnA concentration in fast muscle tissue cellular extracts did not vary between the three experimental groups. (B) CnB nuclear localisation was significantly higher in the E₁₅ and E₃₀ groups relative to the TR group (P<0.01, Kruskal Wallis; ^*P<0.05, ^aP<0.1, post-hoc multiple comparisons). Overall CnB concentration in fast muscle tissue cellular extracts was invariant between the three experimental groups. (C) NFAT2 nuclear localisation was significantly reduced in the E₁₅ and E₃₀ groups relative to the TR group (P<0.001, one-way ANOVA; ^**P<0.01, ^***P<0.001, Tukey's test). Overall NFAT2 concentration was significantly lower in the E₁₅ and E₃₀ groups relative to the TR group (P<0.001, one-way ANOVA; ^***P<0.001, Tukey's test). Data are mean group optical density (± S.E.M.) expressed as a percentage of the TR group. TR and E₁₅, N=9; E₃₀, N=6.