Fig. 1. Antibody screening. (A-C) The Santa Cruz human-specific CnA (sc-9070), Calbiochem mouse-specific CnB (PC-359) and Santa Cruz human-specific NFAT2 antibodies (sc-1149-R, K18-R) were screened against nuclear protein extracts. The CnA, CnB and NFAT2 antibodies each positively detected a single protein of the expected molecular mass (55 kDa, 19 kDa and 103 kDa, respectively) in the mammalian positive controls (M, mouse and R, rat) and rainbow trout (T) nuclear protein extracts. (D) Three forms of the myostatin protein were detected in total fast muscle tissue cellular extracts of the rainbow trout (T) and Atlantic salmon positive control (S): the precursor protein (PC, 53 kDa), latency-associated peptide (LAP, 40 kDa) and the mature peptide (M, 17 kDa). (A,B,D) Ma, Santa Cruz markers (sc-2035); (C) Ma, Bio-Rad high molecular mass markers (161-0311).