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Fig. 2. Stopped-flow reduction of NMDAR current in EAAT3+ oocytes. NMDAR was activated continuously (dashed line) by a solution containing 3 µmol l-1 glutamate. Superfusate flow was stopped (solid line) for about 15 s during the activation. (A) Glutamate-induced current was decreased when the flow was stopped in EAAT3+ oocytes, (B) whereas the interruption of the flow had no effect on glutamate-induced current in EAAT3- oocytes. Restarting the flow for 5 s restored the full amplitude of the glutamate-induced current in EAAT3+ oocytes.