Fig. 9. NADPH diaphorase staining and citrulline immunostaining in the polyp anthocodium and the colonial mass. (A) Whole mount of tentacle/oral disk region showing citrulline immunoreactivity in basiectodermal cells (arrowheads). (B) Cross section through two tentacles showing bands of citrulline immunoreactivity at the ectoderm/mesoglea and at the mesoglea/endoderm boundaries (arrows). (C) Citrulline immunoreactivity (arrows) at the interface of body wall (parietal) and septal muscle in a whole-mount of polyp tissue. (D) Whole mount of lower polyp body column displaying NADPH diaphorase staining in circular muscle of the body wall (arrows) and in the muscle sheet of a flattened septum (between arrowheads). (E) Whole mount of circular muscle sheet inside the colonial mass in which both multipolar cells (arrowheads) and muscle feet (arrows) show citrulline immunoreactivity. (F) Section through the colonial mass in which are embedded the endodermal muscles of the submerged part (zooecium) of the polyps. Note the citrulline immunostaining in the septal (radial and longitudinal) muscles and in the circular muscle. Scale bars: 30 µm (A,C), 50 µm (B), 100 µm (D), 10 µm (E) and 40 µm (F).