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Fig. 2. Phylogenetic analysis of rainbow trout cytoplasmic carbonic anhydrase (TCAc) and other {alpha}-CA isozymes. The phylogenetic tree was constructed using neighbour joining analysis with support for nodes assessed using bootstrap analysis. The tree was ordered using mouse, rat and human CA VII as a monophyletic outgroup. Branches are drawn to scale with the length of 0.1 approximating replacement of 10% of the amino acids in the protein alignment (no Poisson correction for multiple hits).