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Fig. 4. Total power (WT) (A), steady power (WS) (B) and oscillatory power (WO) (C) in HH21 (WT: P<0.01; WS: P<0.01; WO: P<0.01) HH24 (WT: P=0.02; WS: P=0.02; WO: P=0.03) and HH27 (WT: P=0.40; WS: P=0.41; WO: P=0.42) control and experimental groups. All power parameters significantly declined post-ligation in LAL groups, reflecting a decrease in preload. However, by HH24, power parameters became more similar to CON values and normalized by HH27. Power parameters also decreased in VAL embryos after ligation and normalized by HH27. Symbols and abbreviations described in Fig. 2. Data are mean ± S.E.M.