Fig. 2. Morphological consequences for in vitro maintenance of extirpated buds and migrating cells. (A) Aggregations of hemocytes released from an isolated buds, day 7 of culture (x200). (B) Test cells attached to the plate (arrows) after migrating from an isolated bud, day 3 of culture (x200). (C) A hollow sphere development from an extirpated stage `B' bud (x200). (D) Epithelial monolayer originating from isolated and cultured buds, in 14 day-old culture (x100). (E) Phase contrast photomicrographs of 1-month old culture, with confluent epithelial monolayer grown from a bud isolated at stage `D', showing cuboidal cell morphology with a polygonal shape (x400). (F) A deteriorating monolayer, day 21 (x200). b, bud body; n, nucleus; pc, pigment cell; s, sphere; vc, vacuolated cell. Bars, 50 µm.