Fig. 4. Chronograms illustrating the daily rhythmic pattern of CRY abundance in P. clarkii. Bars at the bottom of each panel indicate the illumination conditions (open, light; closed, dark). Values are means ± S.D. (N=3). The corresponding western blot is shown at the top of each panel. Each lane represents the time points of sample collection. D-CRY is the stained control peptide. (A–C) Changes in the daily pattern of CRY abundance in the eyestalk, showing no significant circadian rhythms. (D–F) Changes in the rhythmic pattern of CRY abundance in the brain of P. clarkii: when the organisms changed from LD to DD, a clear circadian pattern was observed. Note that CRY abundance rhythm mean (mesor) increases under continuous darkness, especially in the eyestalk. The data for B,C,E,F were obtained from tissues taken from animals maintained in the dark for 24 and 72 h, respectively. A and D show data obtained from tissues form animals maintained in LD. Relative CRY abundance is indicated by the relative average intensity of the immunoreactive area of the band.