Fig. 8. Accumulated mRNA levels of mWap65-1 and mWap65-2 in livers from adult medaka acclimated to 10 and 30°C. (A) Northern blot analysis was performed for four groups each containing three individuals acclimated to either 10 or 30°C. Lanes 1–4 contained 20 µg of total RNAs from the liver of fish acclimated to 10°C, and lanes 5–8 contained those from each group of fish acclimated to 30°C. (B) The transcriptional levels of mWap65-1 and mWap65-2 relative to those of 18S rRNA. RNA blots for mWap65-1 and mWap65-2 were quantified using a Fujix BAS 1000 densitometer, and signal intensities of 18S rRNA in agarose gels stained with ethidium bromide were quantified by using the Electrophoresis Documentation and Analysis System 120. Relative mRNA levels are compared to those of 18S rRNA for the same sample; values are means ± S.D.