Fig. 7. Quantitative RT-PCR analyses on mRNAs encoding mWap65-1 and mWap65-2, and ß-actin from embryos at various developmental stages (h.p.f.). (A) Agarose gel electrophoresis patterns for amplified products obtained by RT-PCR. Total RNAs were isolated from embryos at different developmental stages (see Table 1). The arrow indicates the stage at which the liver begins to develop. After PCR, amplified products were separated on agarose gels and stained with ethidium bromide. Numbers in the left margin represent base pairs of X174 DNAs digested with HincII. (B) Changes in relative transcription levels of mWap65-1 and mWap65-2 during ontogeny. The signal intensities of each transcript in agarose gels stained with ethidium bromide were quantified, using Electrophoresis Documentation and Analysis System 120, relative to those of ß-actin at the same stage.