Fig. 5. SDS-PAGE (A) and immunoblotting (B,C) patterns of blood solution fractions obtained by hemin–agarose affinity chromatography. Immunoblotting used antisera specific to mWap65-1 (B) and to mWap65-2 (C). Lanes 1, blood solution of 2.8 mg ml^–1 obtained from adult medaka; lanes 2, supernatant; lanes 3, eluted fraction; lanes 4, proteins still bound to hemin–agarose after elution; M, molecular mass markers (kDa). 10 µl solution/lane. Arrowheads, see Fig. 4.