Fig. 4. Ferritin heavy chain translation status measured using polysome profiles of L. littorea hepatopancreas extracts prepared using post-mitochondrial supernatants centrifuged on 15%–30% continuous sucrose density gradients. Fractions were collected with high sucrose (30%) in fraction 1, decreasing to 15% in fraction 15. Bar graphs show absorbance at 254 nm, representing the relative amount of total RNA in each fraction. P, polysomes; M, monosomes. Line graphs show ferritin heavy chain transcript levels as determined from northern blots of total RNA isolated from each fraction. (A) normoxia; (B) 72 h anoxia; (C) 6 h aerobic recovery following 72 h anoxia.