Fig. 2. Confocal scanning of yolk-sac mitochondria-rich cells (MRCs) double-labeled with Con-A–Texas-Red (red) and Na⁺/K⁺-ATPase antiserum (second antibody conjugated with FITC; green) in larvae. The densities of MRCs (A) and their exposed apical surfaces (B) were separately quantified, and the ratio of active MRCs over total MRCs was calculated as described in the Materials and methods. Scale bars, 50 µm.