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Fig. 3. Glycerol gradient velocity sedimentation of wild-type CSPs (cDNA1), CLP, SSP, CSLP, L{Delta}8 and C{Delta}27 in null-mutant (U1) and wild-type (WT) backgrounds. S, supernatant; P, pellet, SP soluble proteins; SV, synaptic vesicles; PM, plasma membrane. The gradient was allowed to develop overnight from 5% to 25% in 5% steps. Wild-type CSPs migrate in the synaptic vesicle fractions whereas SSP and CLP comigrate with the soluble control protein SAP47. A significant portion of CSLP, L{Delta}8 and C{Delta}27 appears to comigrate with the plasma membrane fraction identified by the PM protein syntaxin (SYX).