Fig. 2. (A) Ni concentrations in various tissues of juvenile rainbow trout following 42 days of exposure to either control (open bars), 384 µg Ni l^–1 (hatched bars) or 2034 µg Ni l^–1 (filled bars). Values are means ±1 S.E.M. (N=6–10). Asterisks indicate significant difference (P<0.05) from control mean by a one-way ANOVA with a two-sided Dunnett's post-hoc multiple comparison test. (B) Relative distribution of accumulated Ni among eight tissues in a hypothetical 1-kg rainbow trout. Accumulated Ni in each tissue (µg Ni kg^–1 tissue; see A) was multiplied by that tissue's relative proportion of total body mass to normalize individual tissue Ni burdens to a hypothetical 1-kg fish. Therefore, normalized accumulation is expressed as µg Ni kg fish^–1 for each tissue. (C) Percentage of accumulated tissue Ni burdens that can be explained purely by Ni within the blood plasma perfusing each tissue (see Discussion for details).