Fig. 7. Normal expression pattern of Broad (BR) proteins in the prothoracic gland (PG) cells. Third instar larvae or white puparia were dissected at designated times (hours after egg laying). Each ring gland was double stained with antibodies against BR core region and one of the BR isoforms as described in Materials and methods. Note that the anti-BR-Z2 antibody also nonspecifically stained the nucleoli.