Fig. 6. Ectopic expression of Broad (BR) proteins in the prothoracic gland cells of the ring gland causes degeneration. (A) Expression of BR-Z3 in the ring gland driven by P0206 caused degeneration of the entire PG portion of the ring gland in a prolonged second instar larva (48 h after ecdysis into the second instar; right). Only part of the corpora allata (CA) remained intact (arrowhead). Compare to an intact ring gland of a second instar control larva (left). (B) The process of degeneration of the prothoracic gland (PG) cells was visualized by expressing both green fluorescent protein (GFP) and BR isoforms via P0206. The ring glands are outlined by broken lines, and the arrows point to the CA. Scale bar, 25 µm. Abbreviations: br, brain; ca, corpora allata; ed, eye disc; pg, prothoracic gland cells.