Fig. 2. Broad (BR) protein expression pattern after heat induction of BR-Z3 at 5 h of the second instar. (A) BR proteins were detected in all tissues by an anti-BR core region antibody at 12 h after the heat shock. (B) At 18 h only trace amounts of BR protein remain in the fat body. BR is also found in certain cells in the CNS, as in normal second instar larvae (F). (C) By 24 h after the heat shock, BR proteins were only detected in the CNS, as in the normal second instar larva (F), indicating that all heat-induced BR proteins were catabolized by that time. (D) A new round of br expression began around 36 h after the heat shock, and (E) high levels of BR were seen in the imaginal discs at 72 h. Abbreviations: ad, antenna disc; br, brain; cns, central nervous system; ed, eye disc; epd, epidermis; fb, fat body; ld, leg disc; sg, salivary gland; wd, wing disc.