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Fig. 7. Semi-quantitative northern blot analysis: MPII mRNA levels were unchanged regardless of intoxication in control (A), acute (B) or chronically (C) intoxicated worms. (A) Northern blot of 18S and MPII RNA (see Materials and methods). (B) Density quantification of MPII RNA content after analysis using Quantity One software (Bio-Rad), relative to 18S expression level.