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Fig. 6. Expression of hsp23, hsp70, hsp90 and hsc70 transcripts in response to the rehydration of desiccated (A) nondiapausing and (B) day 15 diapausing pupae. C represents RNA from a nondesiccated control. D represents pupae desiccated for 4 days at <5% RH. Remaining lanes represent pupae rehydrated for 1 or 2 days at 75% or 100% RH. Each treatment was run in triplicate. 28s ribosomal RNA was used as a control to confirm equal sample loading.