Fig. 4. Expression of hsp23, hsp70 and hsp90 transcripts during diapause termination in day 15 diapause pupae previously desiccated at <5% RH/25°C for 9 days. C represents RNA from a nondesiccated diapause control sample. D represents a desiccated diapause sample (9 days at <5% RH). Other lanes represent hours following the termination of diapause elicited by an application of hexane. Each treatment was replicated at least twice. 28s ribosomal RNA was used as a control to confirm equal sample loading.