Fig. 3. Expression of hsp23, hsp70, hsp90 and hsc70 transcripts in (A) day 15 and (B) day 30 diapausing pupae. The puparium was opened in all cases. C represents RNA from a nondesiccated, nondiapausing control, and other lanes indicate the number of days that diapausing pupae were exposed to desiccation (<5% RH/25°C). Each sample was run in triplicate. 28s ribosomal RNA was used as a control to confirm equal sample loading.