Fig. 2. Expression of hsp23, hsp70, hsp90 and hsc70 transcripts in nondiapausing pupae in response to desiccation. Pupae were encased in either (A) opened or (B) intact puparia. Lanes represent RNA samples from pupae desiccated at <5% RH/25°C for 0–6 days or exposed to a heat shock (HS) of 1 h at 40°C. Each sample was run in triplicate. 28s ribosomal RNA was used as a control to confirm equal sample loading.