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Fig. 2. Bafilomycin (50 nmol l–1)-sensitive H+-ATPase activity in the total cell fraction [i.e. cells collected from combined PVC/MR (pavement cell/mitochondria-rich) fraction, the 1.03–1.09 g ml–1 Percoll interface]. Freshwater control fish compared with both 10{per thousand} and 30{per thousand} seawater-adapted fish. Significant differences are indicated by different letters. Values represent means ± S.E.M. (N=4–6 fish).