Fig. 1. Elution profile of heparin–Sepharose affinity chromatography column illustrating the elution of protein (absorbance at 280 nm;solid symbols) and the concentration of NaCl in the eluate (open symbols). Typical results from a single experiment. Horizontal bars indicate those fractions with detectable lipase activity against TAG substrates. Activity eluting early in the column is HSL, which is not retarded by the heparin affinity column. Activity eluting later in the chromatogram at high salt concentration is LPL, which is retarded by the heparin affinity column.