Fig. 3. Diuretic hormone 44 (DH) activation of CG8422-expressing HEK-293 cells produces a dose-dependent increase in CRE-luciferase activity. Dose-response curve for DH activation of CRE-luc gene expression in cells transiently co-expressing CG8422. The calculated EC₅₀ is 1.47 nmol l^-1. Values are means ± S.E.M. and represent the results pooled from three experiments that were performed in triplicate. Values observed with exposures to DH above 5x10^-10 mol l^-1 were statistically different from values observed with exposure to vehicle alone.