Fig. 5. RT-PCR analysis of the expression of the OHSS gene. PCR products of 27 cycles of RT-PCR amplification using OHSS cDNA were loaded in 0.8% agarose gel. RNAs were extracted from embryos detached from the female (Emb), the zoea larvae (Zoea), and muscles (FM), hepatopancreas (FH), ovigerous setae (FOs) and brain (FB) of the adult female. Numbers below Emb and Zoea indicate days before (–) and after (+) hatching, and the day of hatching (h). Lane G represents the products of PCR using genomic DNA from embryos as template. Size standards in kb are shown on the left.