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Fig. 4. In vitro effect of estradiol (E2; 10-6 mol l-1) and E2 given in combination with melatonin (mel; 10-6 mol l-1, 10-9 mol l-1 or 10-12 mol l-1) on the mast cell number after 6 h of incubation in testis of frogs captured in October. Vertical bars indicate means ± S.D. Different letters (a versus b) indicate significance at P<0.01.