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Fig. 2. In vitro effect of estradiol (E2; 10-6 mol l-1), E2 given in combination with melatonin (mel; 10-6 mol l-1), and melatonin alone (10-6 mol l-1) on mast cell number (MCN) after 6 h or 12 h ofincubation in testis of frogs captured in October. Values are expressed as means ± S.D. Different letters (a versus b) indicate significance at P<0.01.