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Fig. 4. Tissue distribution of Atlantic cod GLUT1. (A) Northern blot analysis of total RNA from various cod tissues hybridized with a 664 bp antisense RNA probe specific for Atlantic cod GLUT1. Top panel shows the 4560 bp GLUT1 mRNA transcript; bottom panel the 18S rRNA bands, as detected by ethidium bromide staining. MW, RNA marker (kb). (B) RT-PCR analysis. Top panel shows a 642 bpfragment amplified using primers located within the Atlantic cod GLUT1 ORF; bottom panel a 617 bp fragment amplified using cod ß-actin-specific primers. MW, DNA marker (bp).