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Fig. 7. Effects of 5-HT (A), DA (B), OA (C), ACh (D) and Glu (E) on heartbeat of the isolated heart. The agents were applied between the arrows. Heart rate just before 5-HT, DA, OA, ACh, or Glu treatment was 32.6±5.9, 44.6±9.9, 48.2±4.6, 33.8±3.9 or 36.0±4.9, respectively. Graphs show dose–response curves for the agents (mean ± S.D., N=5). As the effects of 5-HT appeared biphasically, inhibition (1st phase) followed by excitation (2nd phase), they are plotted separately.