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Fig. 5. Chaperone protection by {alpha} crystallin of heat-aggregated {gamma} crystallin (A–C) along with protection of DTT-denatured lysozyme (D–F). (A,D) D. mawsoni; (B,E) T. obesus; (C,F) B. taurus. Temperatures for the assay are indicated in each panel. Values are means ± S.E.M. (N=3). In A–C the {alpha} crystallin and {gamma} crystallin are both from the same species, with fractional amounts of {alpha} crystallin added based on a 1:1 mass ratio of {alpha} to {gamma} crystallin at a final assay concentration of 1 mg ml–1 for both crystallins (i.e. x in A–C). In D–F the amount of {alpha} crystallin added was based on a 1:1 mass ratio of {alpha} crystallin to lysozyme, where in all assays (D–F) there was 0.2 mg ml–1 of lysozyme containing 20 mmol l–1 DTT. For A–C: x {gamma}; x 1:16 {alpha}:{gamma}; x 1:8 {alpha}:{gamma}; x 1:4 {alpha}:{gamma}; x 1:2 {alpha}:{gamma}; x {alpha}:{gamma}. For D–F: x 0:1 {alpha}:lyso; x 1:1 {alpha}:lyso; x 2:1 {alpha}:lyso; x 3:1 {alpha}:lyso; x 2.5:1 {alpha}:lyso; x 5:1 {alpha}:lyso; x 7.5:1 {alpha}:lyso; x 10:1 {alpha}:lyso; x 11:1 {alpha}:lyso.