Fig. 4. Determination of the upper limit of thermal stability T_S for the (A) and (B) crystallins from D. mawsoni, measured as the change in turbidity (A₃₆₀) over time at various incubation temperatures. Concentration of crystallins was 1 mg ml^–1 in all assays. (A) Upper limit of T_S for crystallin. At temperatures above 47°C, turbidity increases with time. at 47°C (x); 55°C (x); 60°C (x). (B) Upper limit of T_S for crystallin. at 30°C (x); 33°C (x); 35°C(x); 42.5°C (x); 44°C (x); 45°C (x); 47°C (x); 57.5°C (x). The T_S was determined as 47°C for the and 33°C for the crystallin.