Fig. 6. Semi-quantitative reverse transcriptase/polymerase chain reaction (RT-PCR) analysis of Na⁺/K⁺-ATPase -subunit and V-type H⁺-ATPase B-subunit mRNA abundance in gill homogenates from Dilocarcinus pagei. In all experiments, PCR was performed using 1 µl cDNA, which was 5% of the cDNA reverse transcribed from 2 µg total RNA. The primer pairs NAK 10F/NAK 16R and DiloHATF1/DiloHATR1 were employed to amplify the Na⁺/K⁺-ATPase -subunit and V-type H⁺-ATPase B-subunit, respectively. (A) Demonstration of template-dependent quantification of Na⁺/K⁺-ATPase -subunit (left panels) and V-type H⁺-ATPase B-subunit (right panels) in anterior and posterior gills of D. pagei. (B) Graphs corresponding to data in A, showing the digitized pixel densities of the PCR products. Blue diamonds, anterior gills; red circles, posterior gills. Values are means ± S.E.M. (N=3).