Fig. 1. Agarose gel showing template-dependent, product abundance during the logarithmic phase of PCR amplification. 2 µg total RNA, isolated from the posterior gills of Dilocarcinus pagei, were employed in the reverse transcription assay. Different volumes (0.25, 0.5 or 1 µl) of the resulting cDNA were used to perform 23 PCR amplification cycles. Lane M is a DNA ladder.