Fig. 8. Real-time measurement of the [Ca²⁺]_i response in S2 cells expressing D. melanogaster LKR, CG10626. S2 cells were co-transfected with the D. melanogaster LKR, CG10626 ORF (Radford et al., 2002) and apoaequorin ORF constructs, and expression induced. Data are expressed as [Ca²⁺]_i (nmol l^–1) against time (s); measurements were taken at 0.1 s intervals. The traces shown are average responses (N=5). Peptide was injected at 15 s. (A–C) Samples were stimulated with either Anopheles leucokinin I (A), II (B) or III (C) peptide at a concentration of 10^–6 mol l^–1 (blue) or 10^–7 mol l^–1 (red). (D) Comparison of the responses to the three Anopheles leucokinin peptides applied at a concentration of 10^–6 mol l^–1. Error bars are negligible for all panels.