Fig. 3. The cDNA and putative protein sequence of the Anopheles stephensi leucokinin receptor gene. Nucleotide and amino acid residue numbers are indicated at the end of each line, amino acids are centred on their codons. The polyadenylation signal, and start and stop codons are marked in bold type. Upstream in frame stop codons are double underlined, and the positions of predicted introns in the cDNA sequence are indicated by ><. Predicted TM regions are indicated by a shaded box. The peptide epitope used to raise the anti-CG10626 antibody (Radford et al., 2002) is not present within the A. stephensi receptor sequence. Potential N-glycosylation sites in the protein sequence. The conserved GPCR Asp-Arg-Tyr/His (DR^Y/_H) motif just after TM III and conserved Cys (C) residues in extracellular loops 1 and 2 are also marked in bold type. The position of binding sites for primers used in the analysis and construction of the cDNA sequence are indicated. Nucleotides that are different between the cDNA and primer sequences are indicated in red type.