Fig. 1. The cDNA and protein sequences of the putative Anopheles gambiae preproleucokinin gene. Nucleotide and amino acid residue numbers are indicated at the end of each line, amino acids are centred on their codons. The start codon, following stop codon and putative polyadenylation signal are indicated in bold type. Upstream in-frame stop codons are double underlined. A putative TATA-box sequence is underlined, the initiation of transcription consensus sequence is indicated by a shaded box, and the position of the single intron is indicated by ><. Within the putative preproleucokinin the location of the three A. gambiae leucokinin peptides are indicated by shaded boxes and a possible signal peptide is underlined. Proteolytic processing sites thought to be used are underlined. The Gly (G) residues that are presumed to be processed to C-terminal amides in the mature A. gambiae leucokinins are indicated in bold and double underlined. Four Cys (C) residues within the preproleucokinin are also indicated by shaded boxes.