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Fig. 9. High magnification confocal imaging of CA (green) actin (red) and DNA (blue) in the AMG of a fourth instar Ae. albopictus larva. (A–D) A maximum projection of a z-stack of images showing muscle fibers which are clearly labeled for CA (green in A and yellow in the overlay D) and ones that do not label for CA (only red in D). (E,F) Selected planes of focus from the same z-stack in three-color overlay. Note CA labeling (green) outlines the CA-positive muscles which are internally red, supporting a cell surface CA localization. All images are the same magnification so the magnification bars shown in A–D (20 µm) apply to all images.