Fig. 7. Immunofluorescence and confocal imaging of CA antibody labeling. (A–C) An isolated midgut from a fourth instar Ae. aegypti larva immunostained for CA (A, green) and labeled with TRITC-conjugated Phalloidin (B, red) to visualize actin. (C) Merge of the two colored images. Note the specific checkerboard arrangement of CA labeling in AMG (A, arrows), which corresponds to a subset of the phalloidin labeled muscles seen in B and C. (D–F) A similar subset of the gut musculature at higher magnification, labeled for CA from a fourth instar larva of An. gambiae. Scale bars 200 µm (A–C); 80 µm (D–F).