Fig. 4 The anatomy of the thoracic region and stomach of crayfish. (A) Angled view
of a 3D model reconstructed from MRI data. The anatomical structures
identified and displayed within the thoracic region are the bilateral
antennary arteries (AnA), the ventral artery (VA), the antennal glands (AG),
the anterior part of both lobes of the hepatopancreas (He) and the foregut,
including the stomach (St) and esophagus (Es). The exoskeleton was made
transparent for better illustration. (B) A side view of the same model.
Cutaway of antennal gland and added transparency to the hepatopancreas reveal
internal structures. The bladder (Bl) almost completely encloses the remaining
tissue (iAG) of the antennal glands. The hepatopancreatic tubules (HeT) inside
the hepatopancreas and the duct that connects them to the stomach can also be
seen. Insets in A and B: Red boxes indicate the area displayed beneath in the
enlargements. (C) Sagittal section of the stomach. The internal structures
identified and displayed within the foregut are the esophagus (Es), the
cardiac stomach (CSt), the lateral (LT) and medial tooth (MT), the
ventrolateral cardiac filter (VCF), the cardio-pyloric valve (CpV), the
pyloric stomach (PSt) and the dorsal valve (DV). (D) Coronal section of the
(cardiac) stomach. Identified and displayed structures are the lateral teeth
(LT), the esophagus (ES) and the cardio-pyloric valve (CPV). The external
tissue surrounding the interior of the stomach is displayed as a uniform
structure in bright yellow color. Other organs were removed for means of
clarity. Insets in C and D: Dark planes indicate the positions at which the
portions of the crayfish displayed beneath in the enlargements were cut open.
All scale bars, 1 cm. Data in AD were reconstructed from images
acquired in the same crayfish (mass 31.8 g) that was injected with 60 µl of
a 120 mmol l1 MnCl2 solution and scanned at
TR=1.5 s, TE=17.6 ms; matrix
dimensions: 256x256, field of view=5.12 cmx5.12 cm, number of
averages=20, slice thickness, 200 µm; voxel size, 200x200x200
µm; acquisition time (total), 12.5 h.