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Fig. 2. Effects of injection with saline or E. coli LPS on brain and pituitary tissue CRH levels in tilapia (N=6 in all cases). Open bars indicate pre-confinement values and solid bars indicate values of fish sampled following 24 h confinement. The asterisks (*) indicate confinement effects within a saline- or LPS-treatment group (see Presentation of data and statistics for details). Upper left panel: sagittal overview of the brain of tilapia showing the dissected parts (telencephalon, diencephalon, rhombencephalon and pituitary). The grey areas indicate brain parts that were discarded: tectum and midbrain, and the rostral part of the spinal cord.